Cell freezing or cryopreservation is the method of freezing tissues and cells at low temperatures to keep the biological material metabolically inert and genetically stable, and to reduce ice crystal formation. Ice crystals are smaller crystals of ice that form when the temperature is quite low and these can disrupt the cell membrane of the cell or tissue and can lead to death of cell. Cryopreservation is done to exchange some of the water cells with other compounds to reduce crystal formation. For cell freezing, various types of media or serum are used. The most common substitutes are glycerol and DMSO (dimethyl sulfoxide). Glycerol and dimethyl sulfoxide are mixed into a solution with serum or media in which cells are sited and suspended in a liquid nitrogen freezer. As media starts to freeze, the concentration of salt outside the cells become greater than the cells, and water leave the cells to exchange by cryopreservative.
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Cell freezing media commonly comprises a protein source, base medium, and a cryopreservative. Cryopreservatives are used to protect the cells from physical stress, mechanical and diminishes the water content inside the cells, and minimizing the development of cell death or celllysing ice crystals. The protein source also protects the cells from the stress linked with the freeze-thaw process. Cells are frozen gradually, at one degree Celsius per minute, using techniques and programmable coolers.
The cell freezing media market has been segmented based on type of media and end-user. In terms of type of media, the cell freezing media market has been categorized into Chinese hamster ovary (CHO) cell Media, hybridoma media, insect media and vaccine production media. Chinese hamster ovary cells are capable of harvesting various recombinant glycoproteins at great levels on a big scale and have potential in pharmaceutical research and development and in bioprocessing. Hybridoma media is used in the development of monoclonal antibodies in cell culture system. Insect media is used for development of genetically engineered cell lines to express therapeutically valuable recombinant proteins. Insect media is a rapidly growing segment as it has created major breakthrough in health care. The vaccine production media is of various types such as classic media and salts, additional vaccine media, cell line media, fetal bovine serum (FBS), and other animal sera.
In terms of end-user, the cell freezing media market has been segmented into biopharmaceutical companies, pharmaceutical companies, and academics and research. Biopharmaceutical companies is the fast growing segment of the cell freezing media market owing to increasing research in the segment. Cell freezing media are used in pharmaceutical medicines for drug screening, formation of regenerative medicine, etc.
In terms of region, the cell freezing media market has been segmented into North America, Europe, Asia Pacific, Latin America, and Middle East & Africa. North America and Europe are projected to account for major share of the global cell freezing media market. Increasing research activities in North America and Europe for new drug development is anticipated to drive the cell freezing media market during the forecast period. Asia Pacific is the most progressive region for the cell freezing media market due to rising focus on bioprocessing and pharmaceutical research and development in countries such as Japan, China, and India.
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Increasing government initiatives for research in the pharmaceutical and biopharmaceutical segment is expected to fuel the growth of the cell freezing media market. Additionally, advantages of cell freezing for storage in drug development or in other programs are likely to fuel the growth of the market. However, high maintenance cost in storage procedure is the major factor that can hamper the growth of the market during the forecast period.
Major players in the cell freezing media market are Merck KGaA, Thermo Fisher Scientific, Inc., Nippon Genetics, Miltenyi Biotec, Mediatech, Inc., General Electric Company, BioLifeSolutions, Inc., HiMedia Laboratories, and PromoCell GmbH.
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